Dual-Functioning Antifouling Silk Surfaces via Covalent Grafting of a Calcium-Chelating Agent

Дата публикации: 04-08-2026 00:00:00

Bacterial contamination and biofilm formation on material surfaces remain major challenges in USDA-relevant food and agricultural environments, where durable coatings are needed to reduce microbial attachment, persistence, and cross-contamination, including from multidrug-resistant pathogens. Silk fibroin is an attractive coating platform because it is a biocompatible structural protein whose surface chemistry and interfacial behavior can be tailored through chemical modification. In this study, silk fibroin was functionalized with 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) using EDC/NHS coupling to create a phosphonate-modified surface with enhanced ion-binding capability and altered wettability. The resulting silk-HEDP materials were characterized by Fourier transform infrared (FTIR) spectroscopy, water contact angle measurements, and calcium depletion experiments. FTIR confirmed successful incorporation of phosphonate functionality while preserving the characteristic...

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